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Supplemental Data for Wu et al., Cell Stem Cell 1, pp. 541-554
- Document S1. Supplemental Experimental Procedures and Four Figures
- Movie S1. Symmetric Commitment
Representative movie of a GFP+KLSC cell undergoing division followed by downregulation of GFP in both daughter cells (Movie S1). CFP expression serves as a control and does not change significantly after division (Movie S2). Cells of interest are indicated by arrowheads. Clock at bottom of movie represents culture time (hours:minutes) in 10 minute intervals.
- Movie S2. Symmetric Commitment
Representative movie of a GFP+KLSC cell undergoing division followed by downregulation of GFP in both daughter cells (Movie S1). CFP expression serves as a control and does not change significantly after division (Movie S2). Cells of interest are indicated by arrowheads. Clock at bottom of movie represents culture time (hours:minutes) in 10 minute intervals.
- Movie S3. Symmetric Renewal
Representative movie of a GFP+KLSC cell undergoing division followed by maintenance of GFP expression in both daughter cells (Movie S3). CFP expression serves as a control and remains similar in mother and daughter cells (Movie S4). Cells of interest are indicated by arrowheads. Clock at bottom of movie represents culture time (hours:minutes) in 10 minute intervals.
- Movie S4. Symmetric Renewal
Representative movie of a GFP+KLSC cell undergoing division followed by maintenance of GFP expression in both daughter cells (Movie S3). CFP expression serves as a control and remains similar in mother and daughter cells (Movie S4). Cells of interest are indicated by arrowheads. Clock at bottom of movie represents culture time (hours:minutes) in 10 minute intervals.
- Movie S5. Asymmetric Division
Representative movie of a GFP+KLSC cell undergoing division followed by downregulation of GFP expression in one of the daughter cells (Movie S5, bottom arrow). CFP expression serves as a control and remains similar in mother and daughter cells (Movie S6). Cells of interest are indicated by arrowheads. Clock at bottom of movie represents culture time (hours:minutes) in 10 minute intervals.
- Movie S6. Asymmetric Division
Representative movie of a GFP+KLSC cell undergoing division followed by downregulation of GFP expression in one of the daughter cells (Movie S5, bottom arrow). CFP expression serves as a control and remains similar in mother and daughter cells (Movie S6). Cells of interest are indicated by arrowheads. Clock at bottom of movie represents culture time (hours:minutes) in 10 minute intervals.
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